Interleukin-6 human
SIGMA/SRP3096 - Animal-component free, recombinant, expressed in E. coli, suitable for cell culture
Synonym: Interleukin-6 human; IL-6
MDL Number: MFCD00131403
Product Type: Chemical
The biological activity of IL-6 (Cat. No. SRP3096) was tested in culture by measuring its ability to stimulate the proliferation of IL-6- dependent T1165 cells. The EC50 is defined as the effective concentration that elicits a 50% increase in cell growth.
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| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | ≤1 EU/μg endotoxin, tested |
| mol wt | 20.9 kDa |
| packaging | pkg of 100 μg |
| pkg of 20 μg | |
| potency | ≤2.0 ng/mL ED50 |
| Quality Level | 300  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | -10 to -25°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P05231  |
| Application: | IL-6 human has been used for STAT3 (signal transducer and activator of transcription 3) activation. It has also been used as a standard for detection of IL-6 in Ben-Men-I cells (human WHO grade I meningioma derived, hTERT immortalized cells). |
| Biochem/physiol Actions: | IL-6 (interleukin 6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease, and plasmacytoma/multiple myeloma. |
| Biochem/physiol Actions: | Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. |
| General description: | IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130 (membrane glycoprotein 130). The gene IL-6 is mapped to human chromosome 7p15.3. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kDa protein containing 184 amino acid residues. |
| Other Notes: | PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM |
| Packaging: | 100 μg |
| Packaging: | 20 μg |
| Physical form: | Lyophilized from a 0.2 um filtered solution of phosphate buffered saline (PBS), pH 7.4, and stabilizers. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute the contents using endotoxin free water to a concentration of 100 ug/mL. For lower concentrations, the product should be diluted before use in medium containing at least 0.5% protein (i.e., cell culture medium containing 5–10% serum). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | -10 to -25°C |
| UNSPSC | 12352202 |