KGF human
SIGMA/SRP3100 - Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym: Fibroblast Growth Factor-7; HBGF-7; Keratinocyte Growth Factor
Product Type: Chemical
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | human |
| color | off-white to yellow |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 18.9 kDa |
| packaging | pkg of 10 μg |
| potency | ≤10 ng/mL ED50 |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P21781   |
| Biochem/physiol Actions: | Fibroblast growth factor (FGF) family members are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. This protein associates with and activates fibroblast growth factor receptors (FGFRs). Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, re-epithelialization of wounds, hair development and early lung organogenesis. |
| General description: | Fibroblast growth factor-7 (FGF-7) is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities. The gene encoding this protein is localized on human chromosome 15q21.1. |
| Physical form: | Lyophilized from 15 mM Sodium Phosphate pH 7.0 + 0.25 M NaCl. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in 1x PBS to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C. |
| Sequence: | MCNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |