MMP-1 human
SIGMA/SRP3117 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: Fibroblast collagenase
Product Type: Chemical
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | human |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 42.7 kDa |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P03956   |
| Application: | MMP-1 (matrix metalloproteinase 1) human has been used to study effect of NF (nuclear factor)κB-mediated expression of MMP-1 in breast cancer spheroids. |
| Biochem/physiol Actions: | Matrix metalloproteinases (MMPs) enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens, aggrecan, serpins, and α2-macroglobulin. It is overexpressed in lumbar intervertebral disc degeneration (IDD). It might also be associated with osteoarthritis. MMP-1 is linked with Jjuvenile idiopathic arthritis. Genetic variations in MMP-1 can affect the susceptibility and severity of this disease. |
| General description: | Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. MMP-1 is expressed in various cells, including fibroblasts, keratinocytes, chondrocytes, monocytes and macrophages, hepatocytes and many tumor cells. The gene is mapped to human chromosome 11q22. Recombinant human MMP-1 is a 42.7kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain which is involved in substrate specificity and in binding TIMP-1 (tissue inhibitor of metalloproteinases 1). |
| Physical form: | Lyophilized from 20 mM TRIS, pH 9.0 + 0.1 mM Calcium Chloride + 100 mM Arginine. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Sequence: | MFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEKAF QLWSNVTPLT FTKVSEGQAD IMISFVRGDH RDNSPFDGPG GNLAHAFQPG PGIGGDAHFD EDERWTNNFR EYNLHRVAAH ELGHSLGLSH STDIGALMYP SYTFSGDVQL AQDDIDGIQA IYGRSQNPVQ PIGPQTPKAC DSKLTFDAIT TIRGEVMFFK DRFYMRTNPF YPEVELNFIS VFWPQLPNGL EAAYEFADRD EVRFFKGNKY WAVQGQNVLH GYPKDIYSSF GFPRTVKHID AALSEENTGK TYFFVANKYW RYDEYKRSMD PGYPKMIAHD FPGIGHKVDA VFMKDGFFYF FHGTRQYKFD PKTKRILTLQ KANSWFNCRK N |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |