Neurturin human
SIGMA/SRP3124 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: NRTN; NTN
Product Type: Chemical
assay | ≥98% (HPLC) |
≥98% (SDS-PAGE) | |
biological source | human |
color | white to off-white |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mol wt | 11.8 kDa |
packaging | pkg of 20 μg |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | Q99748 ![]() ![]() |
Biochem/physiol Actions: | Neurturin (NTN) signals through a multicomponent receptor system, composed of RET and one of four GFRα (α1-α4) receptors. Neurturin promotes the development and survival of sympathetic and sensory neurons by signaling through a receptor system composed of RET and GFRα2. NTN modulates spermatogenesis, kidney morphogenesis and controls smooth muscle of penile blood vessels and several intrapelvic organs. |
General description: | Neurturin (NTN) is a disulfide-linked homodimer neurotrophic factor structurally related to glial cell derived neurotrophic factor (GDNF), artemin, and persephin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. The functional form of human Neurturin is a disulfide-linked homodimer, of two 11.8kDa polypeptide monomers (206 total amino acid residues). Each monomer contains seven conserved cysteine residues, one of which (Cys 69) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. |
Physical form: | Lyophilized from 10 mM Sodium Citrate, pH 4.0. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | MARLGARPCG LRELEVRVSE LGLGYASDET VLFRYCAGAC EAAARVYDLG LRRLRQRRRL RRERVRAQPC CRPTAYEDEV SFLDAHSRYH TVHELSAREC ACV |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |