NP-1 human
SIGMA/SRP3126 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: Cryptdin; HNP-1; Neutrophil Peptide-1; alpha Defensin-1
Product Type: Chemical
antibiotic activity spectrum | fungi |
mycobacteria | |
viruses | |
assay | ≥98% (HPLC) |
≥98% (SDS-PAGE) | |
biological source | human |
color | white to off-white |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mode of action | cell membrane | interferes |
mol wt | 3.4 kDa |
packaging | pkg of 20 μg |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | O14786 |
Biochem/physiol Actions: | Neutrophil peptide-1 (NP-1) exhibits antimicrobial activity. It also has a chemotactic function in dendritic cells. It has a role in the growth and metastasis of cancer cells. The protein acts as multifunctional co-receptor and interacts with various signaling cascades. NP-1 associates with epithelial growth factor (EGF) and its receptor (EGFR). It also interacts with hepatocyte growth factor (HGF) and its receptor c-Met. |
General description: | Chemical structure: peptide |
General description: | Defensins (α and β) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins which include neutrophil peptides-1, -2 and -3, (NP-1, NP-2 and NP-3) are distinguished from the β-defensins by the pairing of their three disulfide bonds. Neutrophil-1 (NP-1) is expressed as the C-terminal portion of an inactive precursor protein, which also contains a 19 amino acid N-terminal signal sequence and a 45 amino acid polypeptide. The protein contains a six-cysteine motif that forms three intra-molecular disulfide bonds. It is a transmembrane glycoprotein. Recombinant human NP-1 is a 3.4kDa protein containing 30 amino acid residues. |
Physical form: | Lyophilized with no additives. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | ACYCRIPACI AGERRYGYCI YQGRLWAFCC |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352202 |