Osteopontin human
SIGMA/SRP3131 - recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture
Synonym: Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; Uropontin
Product Type: Chemical
| assay | ≥97% (HPLC) |
| ≥97% (SDS-PAGE) | |
| biological source | human |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 60.0-65.0 kDa |
| packaging | pkg of 50 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P10451   |
| Application: | Osteopontin human has been used in in vitro invasion assay to study the effect of endostar on non small cell lung cancer cell invasion. |
| Biochem/physiol Actions: | Osteopontin is a secreted glycoprotein that functions as a ligand to αvβ3 integrin and possibly other receptors. It binds tightly to hydroxyapatite and can act as a structural component of the extracellular mineralized matrix. In addition to its involvement in mineralized matrix formation, Osteopontin can also function as a cytokine that stimulates the release of IFNg (interferon γ) and IL-12 (interleukin 12), while inhibiting the production of IL-10. Osteopontin is involved in inflammation and also regulates immunity against infectious diseases. It also co-stimulates T cell proliferation. Osteopontin is also associated with multiple sclerosis. It participates in inflammation in Crohn′s Disease. Osteopontin plays a crucial role in bone remodelling and suppresses ectopic calcification. |
| General description: | Osteopontin is initially secreted as a 298 amino acid protein, which is subject to multiple post-translational modifications including glycosylation, phosphorylation, and specific proteolytic cleavages into various smaller molecular weight fragments. Osteopontin is expressed in a wide range of cells and tissues including osteoblasts, various tumor cell lines, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta and odontoblasts. Recombinant human Osteopontin is a 298 amino acid protein, which, due to glycosylation, migrates at an apparent molecular weight of 60.0-65.0kDa by SDS-PAGE analysis under reducing conditions. |
| Physical form: | Lyophilized from 10 mM Sodium Phosphate, pH 7.0. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Sequence: | IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (HPLC); ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |