p16-INK-4a human
SIGMA/SRP3134 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym: CDK4I; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; Multiple tumor suppressor 1; p16-INK4; p16INK4A
Product Type: Chemical
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | human |
| color | off-white to yellow |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 16.5 kDa |
| packaging | pkg of 20 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P42771   |
| Biochem/physiol Actions: | Cyclin dependent kinase inhibitor 2A (CDKN2A) or p16-INK4a regulates the cell cycle by inhibiting cyclin dependent kinase-4 (CDK4) and CDK6. It inhibits CDK activity by binding to the CDK molecules in a manner that interferes with their ability to interact with cyclin D. This activity has the effect of suppressing tumor formation and growth, and of inducing replicative senescence in various normal cells, including stem cells. The expression of p16-INK4a steadily increases with age and tends to accumulate in stem cell compartments. The deletion, rearrangement, or mutation of the p16-INK4a gene is frequently found in melanomas as well as in certain other types of cancer. The protein is downregulated in bladder cancer. |
| General description: | p16-INK4a, which is also known as cyclin dependent kinase inhibitor 2A (CDKN2A), is a nuclear protein. The gene encoding this protein is localized on human chromosome 9p21, with three exons. Recombinant p16-INK4a is a 16.5kDa protein containing 156 amino acid residues. |
| Physical form: | Lyophilized from 1 x PBS + 1.0 mM DTT, pH 7.2. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Sequence: | MEPAAGSSME PSADWLATAA ARGRVEEVRA LLEAGALPNA PNSYGRRPIQ VMMMGSARVA ELLLLHGAEP NCADPATLTR PVHDAAREGF LDTLVVLHRA GARLDVRDAW GRLPVDLAEE LGHRDVARYL RAAAGGTRGS NHARIDAAEG PSDIPD |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |