p16-INK-4a TAT human
SIGMA/SRP3135 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym: CDK4I; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; Multiple tumor suppressor 1; p16-INK4; p16INK4A
Product Type: Chemical
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | human |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 18 kDa |
| packaging | pkg of 25 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P42771   |
| Biochem/physiol Actions: | Cyclin dependent kinase inhibitor 2A (CDKN2A), also known as p16-INK-4a, arrests cell cycle progression by inhibiting S-phase and promotes apoptosis. The encoded protein has anti-proliferative biological activity, and is implicated in the retinoblastoma (Rb) protein pathway. p16-INK-4a acts as a tumor suppressor and plays a vital role in ageing and cellular senescence. Increased expression of this protein might lead to various types of high grade cancers. p16-INK-4a also facilitates cell invasion and angiogenesis. Bronchial lavage P16INK4A promoter methylation might function as a potential biomarker for diagnosis of lung cancer. |
| General description: | Cyclin dependent kinase inhibitor 2A (CDKN2A) also known as p16-INK-4a, belongs to the Ink4 (inhibitor of CDK4) family of CDK inhibitors. It is encoded by the gene mapped to human chromosome 9p21 within the INK4a/ alternate reading frame (ARF) locus. Human P16-INK4A is localized in both nucleus and cytoplasm, and its expression increases with ageing in skin and kidney tissues. |
| Physical form: | Lyophilized with no additives. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Sequence: | EPAAGSSMEP SADWLATAAA RGRVEEVRAL LEAGALPNAP NSYGRRPIQV MMMGSARVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLVVLHRAG ARLDVRDAWG RLPVDLAEEL GHRDVARYLR AAAGGTRGSN HARIDAAEGP SDIPDGYGR KKRRQRRR |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |