sFRP-1 human
SIGMA/SRP3154 - recombinant, expressed in HeLa cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture
Synonym: SARP-2; Secreted Apoptosis Related Protein-2; Soluble Frizzled Related Protein-1
Product Type: Chemical
| assay | ≥97% (HPLC) |
| ≥97% (SDS-PAGE) | |
| biological source | human |
| color | white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 31.0-36.0 kDa |
| packaging | pkg of 20 μg |
| potency | 0.3-0.5 μg/mL ED50 |
| recombinant | expressed in HeLa cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | Q8N474   |
| Biochem/physiol Actions: | Secreted Frizzled-related protein 1 (SFRP1) is a secreted inhibitor of Wnt signaling pathway. DNA damage or oxidative stress causes cellular senescence, which leads to overexpression of sfrp1. This protein induces senescence phenotypes via the inactivation of Wnt signaling and activation of the retinoblastoma (Rb) pathway. Mutations or aberrant methylation in this gene impair the induction of senescence in several cancers, including breast cancer, bladder cancer, and colorectal tumorigenesis. |
| General description: | The gene SFRP1 (secreted frizzled related protein 1) is mapped to human chromosome 8p22–p12, a region frequently deleted in breast cancer. It is highly expressed in normal breast tissue. |
| Physical form: | Lyophilized from 10 mM Sodium Phosphate pH 7.0. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Sequence: | SEYDYVSFQS DIGPYQSGRF YTKPPQCVDI PADLRLCHNV GYKKMVLPNL LEHETMAEVK QQASSWVPLL NKNCHAGTQV FLCSLFAPVC LDRPIYPCRW LCEAVRDSCE PVMQFFGFYW PEMLKCDKFP EGDVCIAMTP PNATEASKPQ GTTVCPPCDN ELKSEAIIEH LCASEFALRM KIKEVKKENG DKKIVPKKKK PLKLGPIKKK DLKKLVLYLK NGADCPCHQL DNLSHHFLIM GRKVKSQYLL TAIHKWDKKN KEFKNFMKKM KNHECPTFQS VFK |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (HPLC); ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |