sRANKL human
SIGMA/SRP3161 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: ODF (Osteoclast differentiation factor); TNFSF11; TRANCE (TNF-related activation-induced cytokine); soluble Receptor Activator of NFkB Ligand
Product Type: Chemical
assay | ≥98% (HPLC) |
≥98% (SDS-PAGE) | |
biological source | human |
color | white |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mol wt | 20.0 kDa |
packaging | pkg of 10 μg |
potency | 10.0-25.0 ng/mL ED50 |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | O14788 |
Application: | sRANKL has been used to initiate differentiation of osteoclasts. |
Biochem/physiol Actions: | RANKL (receptor activator of nuclear factor |
General description: | TRANCE (TNF-related activation-induced cytokine), also known as sRANKL (soluble receptor activator of nuclear factor |
Physical form: | Lyophilized from 5 mM Sodium Phosphate, pH 7.6 + 75 mM NaCl. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |