TFF3 human
SIGMA/SRP3169 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: ITF; TFI; Intestinal trefoil factor; TFI; Trefoil Factor 3
Product Type: Chemical
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | human |
| color | white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 13.2 kDa |
| packaging | pkg of 20 μg |
| potency | 1.0-10.0 mg per mL |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | Q07654   |
| Application: | TFF3 human has been used in surface plasmon resonance(SPR) binding analysis to study the molecular interactions between TFF3 and the aminonitrile compound and its effect on inhibition of TFF3-dependent cancer cell survival. |
| Biochem/physiol Actions: | The trefoil factor peptides (TFF1, TFF2, and TFF3) play an important role in intestinal mucosal maintenance, defense, and repair. TFF3 also promotes airway epithelial cell migration and differentiation. TFF3 may be useful as a molecular marker for certain types of cancer. TFF3 interacts with the CD147 receptor to promote migration, invasion, and proliferation in colorectal cancer (CRC), Indicating its involvement in cancer progression. TFF3 stimulates the replication of pancreatic islet β-cells in humans and rodents, leading to increased proliferation while preserving their functionality. The effects of TFF3 on β-cell proliferation have been associated with the activation of the protein kinase B(Akt) and epidermal growth factor receptor (EGFR) signaling pathways. |
| General description: | Researcharea: Cell Signaling The trefoil factor peptides (TFF1, TFF2, and TFF3) are secreted in the gastrointestinal tract. They are secreted peptides which are protease-resistant. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to be expressed in certain cancers, including colorectal, hepatocellular, and in biliary tumors. Recombinant human TFF3 is a homodimeric protein consisting of two 59 amino acid chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds. |
| Physical form: | Lyophilized with no additives. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Sequence: | EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |