TL-1A human
SIGMA/SRP3175 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: TNFSF15; VEGI
Product Type: Chemical
assay | ≥98% (HPLC) |
≥98% (SDS-PAGE) | |
biological source | human |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mol wt | 22.0 kDa |
packaging | pkg of 20 μg |
potency | 10.0-100.0 ng/mL |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | O95150 ![]() ![]() |
Biochem/physiol Actions: | TL-1A belongs to the TNF superfamily of ligands. Recombinant human TL-1A is a 22.0 kDa polypeptide of 194 amino acid residues. |
General description: | TL-1A belongs to the TNF superfamily of ligands. It is expressed predominantly in endothelial cells, and to a lesser extent in placenta, lung, kidney, skeletal muscle, pancreas, small intestine and colon. TL-1A inhibits endothelial cell proliferation and angiogenesis, and has been shown to induce NFkB activation, caspase activity, and apoptosis in responding cell lines. TL-1A interacts with TNFRSF25/DR3 receptor, but can also bind to a decoy receptor TNFRSF21/DR6. Recombinant human TL-1A is a 22.0 kDa polypeptide of 194 amino acid residues. |
Physical form: | Lyophilized from 10 mM Tris, pH 8.5 + 75 mM Arginine. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | QLRAQGEASV QFQALKGQEF APSHQQVYAP LRADGDKPRA HLTVVRQTPT QHFKNQFPAL HWEHELGLAF TKNRMNYTNK FLLIPESGDY FIYSQVTFRG MTSECSEIRQ AGRPNKPDSI TVVITKVTDS YPEPTQLLMG TKSVCEVGSN WFQPIYLGAM FSLQEGDKLM VNVSDISLVD YTKEDKTFFG AFLL |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |