TNF-α human
SIGMA/SRP3177 - Animal-component free, recombinant, expressed in E. coli, suitable for cell culture
Synonym: Cachectin; Cytotoxin; Differentiation-
Product Type: Chemical
The biological activity of TNF alpha (Cat. No. SRP3177) was tested in culture by cytotoxicity assay using L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. EC50 is defined as the effective concentration that causes 50% cytotoxicity.
TNF alpha Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
Apoptosis Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized |
| impurities | ≤1.00 EU/μg endotoxin |
| mol wt | 17.4 kDa |
| packaging | pkg of 50 μg |
| potency | ≤0.10 ng/mL ED50 |
| Quality Level | 300  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01375  |
| Application: | TNF-α (tumor necrosis factor α) human has been used as a positive control for induction of proinflammatory genes in human intestinal and hepatic cell lines. |
| Biochem/physiol Actions: | TNF-α (tumor necrosis factor α) is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. However, it can be a tumor promoter as well as a tumor suppressor. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, apoptosis and diabetes. It is also associated with mood and anxiety disorders. |
| General description: | TNF-α (tumor necrosis factor α) is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. The gene is mapped to human chromosome 6p21.3. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. |
| Other Notes: | VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL |
| Physical form: | Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 0.5mM DTT. |
| Preparation Note: | It is recommended to centrifuge the vial beforereconstitution. A stock solution may be prepared byreconstitution in sterile water to a concentration of0.1 mg/mL. The stock solution may be diluted furtherusing culture media. |
| Preparation Note: | Store the product at −20 °C. After reconstitution, theproduct can be stored at 2-8 °C for up to 1 month.For extended storage, freeze in working aliquots.Repeated freezing and thawing are not recommended.Do not store in a frost-free freezer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |