TSG human
SIGMA/SRP3179 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym: Twisted Gastrulation Protein
Product Type: Chemical
assay | ≥95% (HPLC) |
≥95% (SDS-PAGE) | |
biological source | human |
color | white |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mol wt | 22.2 kDa |
packaging | pkg of 50 μg |
potency | ≤2.0 μg/mL ED50 |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | Q9GZX9 ![]() ![]() |
Biochem/physiol Actions: | Twisted gastrulation protein (TSG) can inhibit bone morphogenetic protein (BMP) activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP4/chordin complex. |
General description: | Twisted gastrulation protein (TSG) is a secreted bone morphogenetic protein (BMP)-binding protein structurally related to the BMP antagonists chordin and noggin. The gene encoding TSG is localized on human chromosome 18p11.22. Recombinant human TSG is a 199 amino acid 22.2kDa protein containing the BMP/TGFb binding portion of the full length TSG protein. |
Physical form: | Lyophilized from 10 mM Sodium Acetate, pH 6.0. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | MCNKALCASD VSKCLIQELC QCRPGEGNCS CCKECMLCLG ALWDECCDCV GMCNPRNYSD TPPTSKSTVE ELHEPIPSLF RALTEGDTQL NWNIVSFPVA EELSHHENLV SFLETVNQPH HQNVSVPSNN VHAPYSSDKE HMCTVVYFDD CMSIHQCKIS CESMGASKYR WFHNACCECI GPECIDYGSK TVKCMNCMF |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |