Vitronectin human
SIGMA/SRP3186 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym: Epiboin; S-Protein; Serum-spreading factor; V75; VTN
CAS Number: 83380-82-9
Product Type: Chemical
Purity Vitronectin human, Cat. No. SRP3186. The purity of human, recombinant Vitronectin expressed in HEK 293 cells (Cat. No. V5890) was assessed on SDS-PAGE (1.25 μg/lane) followed by InstantBlue™ staining (Cat. No. ISB1L) (Representative result).
Bioassay Vitronectin human, Cat. No. SRP3186. The biological activity of human, recombinant Vitronectin (VTN) expressed in HEK 293 cells (Cat. No. SRP3186) was measured in a cell attachment assay using CHO cells. The EC50 is defined as the effective concentration that elicits a 50% increase in cell attachment in a cell-based bioassay.
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | human |
| color | white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 75 kDa |
| packaging | pkg of 250 μg |
| potency | ≤0.5 μg/mL EC50 |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| sterility | sterile |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P04004  |
| Application: | Vitronectin human has been used to study the effects of ECM (extracellular matrix) ligands on C6 astroglioma cells. It has also been used as an attachment-promoting protein for coating the cell plates. |
| Biochem/physiol Actions: | The multimeric Vitronectin can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin αvβ3 enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. Vitronectin is involved in wound repair and also controls complement system. It works as a marker of profibrotic activity in liver, heart and kidney. |
| General description: | Vitronectin is a secreted glycoprotein which is synthesized in the liver. It is also secreted by respiratory epithelial cells and is present in healthy lungs. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers. Recombinant human Vitronectin is a 459 amino acid single chain monomeric protein, which migrates at an apparent molecular weight of 75kDa by SDS-PAGE under reducing conditions. Recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media. |
| Physical form: | Lyophilized from Phosphate Buffered Saline (PBS), pH 7.4, plus 2.5% trehalose. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Sequence: | DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |