Vitronectin human
SIGMA/SRP3186 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym: Epiboin; S-Protein; Serum-spreading factor; V75; VTN
CAS Number: 83380-82-9
Product Type: Chemical
Bioassay Vitronectin human, Cat. No. SRP3186. The biological activity of human, recombinant Vitronectin (VTN) expressed in HEK 293 cells (Cat. No. SRP3186) was measured in a cell attachment assay using CHO cells. The EC50 is defined as the effective concentration that elicits a 50% increase in cell attachment in a cell-based bioassay.
Purity. Vitronectin Human, Cat. No. SRP3186. The purity of human, recombinant Vitronectin expressed in HEK293 cells was visualized on 10% SDS-PAGE (1.25μg/lane) under reducing condition, followed by InstantBlue staining (representative result)
Purity. Human recombinant Vitronectin, Cat. No. SRP3186. was separated on SDS-PAGE and probed with monoclonal anti- Vitronectin antibody (Cat.No. V7881). The primary antibody was developed using Goat anti-mouse IgG (Fab Specific)-Peroxidase secondary antibody (Cat.No. A9917) and a chemiluminescent substrate
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | human |
| color | white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 52.2 kDa (calculated) |
| NCBI accession no. |   |
| packaging | pkg of 250 μg |
| potency | ≤0.5 μg/mL EC50 |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| solubility | water: soluble |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P04004 |
| Application: | Vitronectin human has been used to study the effects of ECM (extracellular matrix) ligands on C6 astroglioma cells. It has also been used as an attachment-promoting protein for coating the cell plates. |
| Biochem/physiol Actions: | The multimeric vitronectin protein can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin αvβ3 enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. It is also involved in wound repair and controls complement system as wel. Moreover, vitronectin functions as a marker of profibrotic activity in liver, heart and kidney. |
| Features and Benefits: | • Human-derived vitronectin • Produced through recombinant expression in HEK 293 cells • Low endotoxin levels |
| General description: | Vitronectin is a secreted glycoprotein which is synthesized in the liver. It is also secreted by respiratory epithelial cells and is present in healthy lungs. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers.The vitronectin gene consists of approximately 4.5–5 kb base pairs with eight exons and seven introns. Recombinant human Vitronectin is a protein consisting of 459 amino acids. It exists in two forms: a single chain and a clipped form consisting of two chains held together by disulfide bonds resulting from an endogenous cleavage. Recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media. |
| Other Notes: | DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL |
| Other Notes: | Under reducing conditions, the single chain migrates at an apparent molecular mass of 75kDa on SDS-PAGE, and the clipped form migrates at molecular masses of 65kDa and 10kDa. |
| Physical form: | Lyophilized from Phosphate Buffered Saline (PBS), pH 7.4, plus 2.5% trehalose. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |