EGF from mouse
SIGMA/SRP3196 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: Epidermal Growth Factor; URG; Urogastrone
Product Type: Chemical
assay | ≥98% (HPLC) |
≥98% (SDS-PAGE) | |
biological source | mouse |
color | off-white to yellow |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mol wt | 6.0 kDa |
packaging | pkg of 500 μg |
potency | 0.1 ng/mL |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | P01132 ![]() ![]() |
Application: | EGF from mouse has also been used in the DMEM/F12 media for growing mouse kidney cells. |
Biochem/physiol Actions: | EGF (epidermal growth factor) has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). EGF plays an important role in embryogenesis and cardiogenesis. |
General description: | EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Recombinant murine EGF is a 6kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide-bonds. |
Physical form: | Lyophilized from 10 mM Sodium Phosphate, pH 8.2. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |