FGF-acidic from mouse
SIGMA/SRP3197 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym: ECGF-beta; FGF-1; Fibroblast Growth Factor-acidic; HBGF-1
Product Type: Chemical
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | mouse |
| color | off-white to yellow |
| form | lyophilized |
| impurities | 0.0344 EU/μg endotoxin, tested |
| mol wt | 15.9 kDa |
| packaging | pkg of 50 μg |
| potency | ≤0.5 ng/mL |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P61148   |
| Application: | FGF-acidic from mouse has been used to induce the expression of PTPIP51 (protein tyrosine phosphatase interacting protein 51) in mouse hippocampal cells. |
| Biochem/physiol Actions: | FGF1 (fibroblast growth factor 1) is mainly involved in cell growth, proliferation and neurogenesis. In high-fat diet (HFD) mice, FGF1 is upreglated in adipose tissue. Absence of FGF1 in mice results in aggressive diabetic phenotype as well as adipose expansion in the presence of HFD. The PPARγ (peroxisome proliferator-activated receptor γ)-FGF1 axis helps in regulating metabolic homeostasis and insulin sensitization in mice. FGF1 also participates in wound healing, post-ischemic heart repair and making of collaterals after hindlimb ischemia. |
| General description: | FGF1 (fibroblast growth factor 1) belongs to the FGF family of genes. In humans, FGF1 is alternatively spliced into four forms: FGF1A (in kidney), FGF1B (in brain), FGF1-C and -D (in vascular smooth muscle cells and fibroblasts). FGF1 interacts with tyrosine kinase FGF receptors (FGFR1-4) with high affinity. It can translocate between the cytosol and nucleus. Recombinant murine FGF-acidic is a 15.9kDa protein consisting of 141 amino acid residues. |
| Physical form: | Lyophilized from 5 mM Sodium Phosphate, pH 7.2 + 100 mM NaCl. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in 5mM Sodium Phosphate, pH 7.0-7.2 to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Sequence: | MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |