IL-3 from mouse
SIGMA/SRP3208 - Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: HCGF; MCGF (Mast cell growth factor); Multi-CSF; P-cell stimulation factor
Product Type: Chemical
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | mouse |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 15.1 kDa |
| packaging | pkg of 10 μg |
| potency | ≤0.05 ng/mL ED50 |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01586   |
| Biochem/physiol Actions: | IL-3 (interleukin 3) plays a key role in the differentiation of murine hematopoietic progenitor cells and the activation and survival of mature myeloid cells. This protein was originally termed as multicolony-stimulating factor (multi-CSF) because of its capability to induce the differentiation of a wide variety of hematopoietic progenitor cells from bone marrow, such as mast cells, basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes. IL-3 promotes mast cell growth, differentiation, and mediator release, hence, suggesting a role in allergic inflammation. In mice, it is involved in suppressing protective immunity against Plasmodium berghei NK651, and prevents the development of splenomegaly, anemia, and erythropoiesis. It functions as a regulator of osteoclastogenesis, where it play a dual role- it facilitate the development of osteoclast progenitors but suppresses the osteoclastogenic process. |
| General description: | IL-3 (interleukin 3) is primarily produced by activated CD4+ T cells and mast cells, and is a multi-lineage hematopoietic growth factor and immunoregulatory cytokine. Recombinant murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues. |
| Physical form: | Lyophilized from 0.1% TFA. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C. |
| Sequence: | MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |