IL-4 from mouse
SIGMA/SRP3211 - Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: B cell stimulating factor (BSF-1); BCDF; BCGF
Product Type: Chemical
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | mouse |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 13.7 kDa |
| packaging | pkg of 20 μg |
| potency | ≤2.0 ng/mL ED50 |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P07750   |
| Biochem/physiol Actions: | It regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 (T helper) cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 (immunoglobulin G1) and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. IL-4 helps in long-term recovery after cerebral ischemia by controlling the activities of microglia/macrophage. In mice, absence of IL-4 is associated with a state anxiety-like behavior. |
| General description: | IL-4 (interleukin 4) is a pleiotropic cytokine. Produced by mast cells, T cells and bone marrow stromal cells. Recombinant murine IL-4 is a 13.7kDa globular protein containing 120 amino acid residues. |
| Physical form: | Lyophilized with no additives. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C. |
| Sequence: | MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |