IL-5 from mouse
SIGMA/SRP3212 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: BCDFII; EDF; TRF
Product Type: Chemical
assay | ≥98% (HPLC) |
≥98% (SDS-PAGE) | |
biological source | mouse |
color | white to off-white |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mol wt | 26.2 kDa |
packaging | pkg of 25 μg |
potency | ≤2.0 ng/mL ED50 |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | P04401 ![]() ![]() |
Biochem/physiol Actions: | IL-5 (interleukin 5) was initially identified as a stimulant of antibody production by activated B cells. It has recently been shown to be a key factor in eosinophil differentiation and proliferation. It is also implicated in the pathogenesis of asthma, atopy, hypereosinophilic syndromes, and parasitic infections. In distal lung, IL-5 recruits eosinophils and modulates inflammatory or immune cells in the microenvironment, thus, promoting lung metastasis in cancer. |
General description: | IL-5 (interleukin 5) is a Th2 (T helper 2) cytokine that is predominantly produced by T lymphocytes, and to a lower extent by mast cells, type 2 innate lymphoid (ILC2) cells, and eosinophils. Recombinant murine IL-5 is a 26.2kDa disulfide-linked homodimeric protein containing two 113 amino acid chains. |
Physical form: | Lyophilized from 10 mM Sodium Phosphate, pH 7.5. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |