TRAIL from mouse
SIGMA/SRP3237 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym: Apo2 Ligand; TL2; TNF-related apoptosis-inducing Ligand; TNFSF10
Product Type: Chemical
assay | ≥95% (HPLC) |
≥95% (SDS-PAGE) | |
biological source | mouse |
color | white |
form | lyophilized |
impurities | <0.1 EU/μg endotoxin, tested |
mol wt | 20.0 kDa |
packaging | pkg of 50 μg |
potency | 40.0-60.0 ng/mL ED50 |
recombinant | expressed in E. coli |
shipped in | wet ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | P50592 ![]() ![]() |
Biochem/physiol Actions: | Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apoptosis is achieved through binding to two death-signaling receptors, death receptor 4 and 5 (DR4 and 5). These receptors belong to the tumor necrosis factor receptor (TNFR) superfamily of transmembrane proteins and contain a cytoplasmic "death domain", which activates the cell’s apoptotic machinery. |
General description: | Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a cytotoxic protein. Recombinant murine TRAIL is a 174 amino acid polypeptide (20.0kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein. |
Physical form: | Lyophilized from 10 mM Sodium Phosphate, pH 7.0. |
Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Sequence: | MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |