Oncostatin-M from rat
SIGMA/SRP3250 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: OSM
Product Type: Chemical
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| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | rat |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 24.4 kDa |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | Q65Z15   |
| Biochem/physiol Actions: | OSM (oncostatin M) functions as a ligand for two high affinity heterodimer receptors that share the signal transducing receptor gp130 (glycoprotein). The two receptors are- type I OSM receptor which is a dimer of the gp130 transducing receptor and the LIF (leukocyte inhibitory factor) receptor β-subunit, and the type II OSM receptor which contains a dimer of gp130 and the OSM receptor β-subunit. OSM is produced by neonatal Sertoli cells, where it induces Sertoli cell and gonocyte proliferation. It is responsible for the induction of spermatogenesis, and negates the effect of luteinizing hormone (LH) on Leydig cell progenitor formation, thus, acting as a regulator for this process. Study in rat shows that this protein stimulates the differentiation of OC15-5 (oval cells) into hepatocytes, and therefore, is thought to be key in liver regeneration by promoting oval cell differentiation into hepatocytes. |
| General description: | OSM (oncostatin M) is a polypeptide growth factor that belongs to the interleukin-6 cytokine family that includes interleukin-6, interleukin-11, leukemia inhibitory factor, ciliary neurotrophic factor, cardiotrophin-1, and novel neutrophin-1/B-cell-stimu Recombinant rat Oncostatin M is a 24.4kDa protein, containing 215 amino acid residues. |
| Other Notes: | MKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP SRSSQSAMLR SLVPR |
| Physical form: | Lyophilized with no additives. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |