SDF-1 alpha from rat
SIGMA/SRP3252 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym: CXCL12; PBSF; Stromal-Cell Derived Factor-1
Product Type: Chemical
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| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | rat |
| color | white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 7.9 kDa |
| packaging | pkg of 10 μg |
| potency | 50.0-100.0 ng/mL |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | Q9QZD1   |
| Application: | Stromal cell-derived factor 1 α (SDF-1α ) has been used in cell migration assay and fibrin gel bead assay. |
| Biochem/physiol Actions: | Stromal cell-derived factor 1 α and β (SDF-1α and β) chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor. SDF-1 α modulates leukocyte and hematopoietic precursor migration and pre-B cell proliferation. |
| General description: | Stromal cell-derived factor 1 α and β (SDF-1α and β) are stromal derived CXC chemokines, and signal through the C-X-C motif chemokine receptor-4 (CXCR4). SDF-1α is also known as C-X-C motif chemokine ligand 12 (CXCL12). Recombinant rat SDF-1α is a 7.9kDa protein containing 68 amino acid residues. |
| Other Notes: | KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK |
| Physical form: | Lyophilized with no additives. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |