NT-3 human
SIGMA/SRP3267 - Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym: HGNF; NGF-2; Nerve growth factor-2
Product Type: Chemical
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | human |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 27.2 kDa |
| packaging | pkg of 10 μg |
| potency | 20-50 ng/mL ED50 |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P20783   |
| Biochem/physiol Actions: | NT3 functions on two structurally unrelated receptor types- the p75 neurotrophin receptor (p75NTR) and the receptor tyrosine kinase (TRK). It promotes the metastatic invasion of brain by breast cancer cells, by promoting the EMT (epithelial-mesenchymal transition) transition of breast cancer cells and suppressing the immune response in brain. This protein is responsible for the indction and survival of neurite outgrowth from a subset of neural crest (NC) and placode-derived neurons. Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kDa polypeptide monomers (240 total amino acid residues). |
| General description: | NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family. This protein is composed of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues. |
| Physical form: | Lyophilized with no additives. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |