PDGF-AA human
SIGMA/SRP3268 - Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym: GDGF; ODGF
Product Type: Chemical
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| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | human |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 28.5 kDa |
| packaging | pkg of 10 μg |
| potency | 1.0-3.0 ng/mL ED50 |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P04085   |
| Biochem/physiol Actions: | The platelet derived growth factors (PDGFs) are stored in platelet a-granules and are released upon platelet activation. They are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. These proteins are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. |
| General description: | Platelet derived growth factors (PDGFs) are disulfide-linked glycoprotein dimers consisting of two 12.0-13.5kDa polypeptide chains, designated as PDGF-A and PDGF-B chains. There are three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5kDa disulfide-linked homodimer of two A chains (250 total amino acids). |
| Physical form: | Lyophilized from 10 mM Acetic Acid. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |