HGF mouse
SIGMA/SRP3300 - recombinant, expressed in Hi-5 Insect cells, ≥95% (SDS-PAGE), ≥95% (HPLC)
Synonym: Hepatopoietin (HPTA); Scatter Factor (SF)
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | mouse |
| color | white to off-white |
| form | lyophilized |
| impurities | <0.1 EU/μg endotoxin, tested |
| mol wt | 80.0 kDa |
| packaging | pkg of 20 μg |
| potency | 10-20 ng/mL |
| recombinant | expressed in Hi-5 Insect cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | Q08048   |
| Biochem/physiol Actions: | Hepatocyte growth factor (HGF) functions via its receptor c-Met, which gets activated upon tyrosine phosphorylation, and subsequently promotes epithelial cell proliferation, morphogenesis, angiogenesis and motility. It plays a crucial role in organ development, as inactivation of either this gene or its receptor in fetal stages results in organ hypoplasia. Endogenously this protein is involved in the self-repair of various ograns including injured livers, kidneys, lungs etc. HGF is a predominant potent factor involved in lung growth and repair. It functions as a motogen, tubulogen and anti-apoptotic agent on airway epithelial and other cells expressing HGF receptor c-Met. Studies in mice show that HGF determines the severity of Pseudomonas aeruginosa keratitis by enhanced HGF signaling through c-met resulting in elevated pro-inflammatory cytokines and reduced anti-inflammatory cytokines. |
| General description: | Hepatocyte growth factor (HGF) is a plasmin related disulfide-linked, hetereodimeric protein containing an α and a β chain. It is synthesized as inactive, single-chain precursor which shares similarity with plasminogen. Its α chain contains an N-terminal hairpin and four kringle domains (NK4). |
| Physical form: | Lyophilized from 10 mM Tris, pH 7.2 + 100mM L-Arginine + 200 mM NaCl. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Note: Slow to dissolve. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |