LIF human
SIGMA/SRP3316 - Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym: D factor; Differentiation-
Product Type: Chemical
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| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | 19.6 kDa |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P15018   |
| Biochem/physiol Actions: | Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts, and inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B-cell lymphoma-extra large) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells. |
| General description: | Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line. Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds. |
| Physical form: | Lyophilized with no additives |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |