EGF from mouse
SIGMA/SRP3329 - Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym: URG; Urogastrone
Product Type: Chemical
Epidermal
Growth Factor Receptor (EGFR) Pathway Research areas:
Cancer biology, Cell proliferation, Metabolism. Disease areas:
Breast, lung, stomach, gastrointestinal cancers, chronic kidney diseases,
glioblastomas.
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | mouse |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | 6.0 kDa |
| packaging | pkg of 500 μg |
| potency | ≥1x10^7 units/mg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P01133   |
| Biochem/physiol Actions: | Epidermal growth factor (EGF) has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with transforming growth factor-α (TGF-α) and VGF (vaccinia virus growth factor). Injection of EGF into neonatal and adult rats has been shown to induce neurobehavioral deficits related to schizophrenia. |
| General description: | Epidermal growth factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. The gene encoding this protein is localized on mouse chromosome 3. Recombinant murine EGF is a 6.0kDa globular protein containing 53 amino acid residues, including three intramolecular disulfide-bonds. |
| Physical form: | Lyophilized from 10 mM Sodium Phosphate, pH 8.5. |
| Preparation Note: | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |