FGF-2 human
SIGMA/SRP4037 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Synonym: FGF-2; Fibroblast Growth Factor-basic; HBGF-2; Prostatropin
MDL Number: MFCD00081382
Product Type: Chemical
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| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | human |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | ~17.2 kDa |
| NCBI accession no. | NM_002006   |
| packaging | pkg of 50 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | P09038 |
| Application: | FGF-2 human has been used: • as medium supplement for E9.5 neuroepithelial cell culture • as a component in the neurobasal growth medium • in growing embryonic neural stem/progenitor cells (NSPCs) in a 2D culture |
| Application: | FGF2 (fibroblast growth factor 2)-human has been used in endothelial colony assay. |
| Biochem/physiol Actions: | FGF2 (fibroblast growth factor 2, also referred to as BFGF (basic fibroblast growth factor)) interacts with FGFR1 (fibroblast growth factor receptor 1), FGFR2 and FGFR3. It stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF2 also determines the fate of neural precursor cells and promotes neuronal axon regeneration. It plays an important role in spinal cord injury repair. FGF2 is known to stimulate angiogenesis and is associated with cancer development. High levels of FGF2 are present in the kidney of children with HIV (human immunodeficiency virus)-renal diseases. The FGF2 mRNA is a target of miRNA miR-503. |
| General description: | The gene FGF2 (fibroblast growth factor 2) is mapped to human chromosome 4q28. FGF-2 is a basic heparin binding growth factor. The recombinant human FGF-2 is a 17.2kDa protein containing 154 amino acid residues. It is also referred to as BFGF (basic fibroblast growth factor). |
| Physical form: | Lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 plus 150 mM NaCl. |
| Preparation Note: | Centrifuge the vial prior to opening. Avoid freeze-thaw cycles. |
| Preparation Note: | Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |