gAcrp30/Adiponectin from mouse
SIGMA/SRP4592 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE)
Synonym: Acrp30; Adipocyte-specific protein AdipoQ; Adipolean; Apm-1; adipoQ; adiponectin
Product Type: Chemical
| assay | ≥98% (SDS-PAGE) |
| biological source | mouse |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | ~16.6 kDa |
| NCBI accession no. | Q60994   |
| packaging | pkg of 25 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| Biochem/physiol Actions: | In obese mice and humans, adiponectin/gAcrp30 (adipocyte complement-related protein) mRNA expression is reduced, therefore linking this hormone to energy homeostasis. In incubated mouse muscle and cultured cells, gAcrp30 leads to an increase in fatty acid oxidation. This hormone is also linked to anti-atherogenesis, and in apoE-deficient mice, overexpression of gAcrp30 results in significant attenuation of atherosclerosis. Long-term administration of this hormone in mice results in weight loss without reduction in food intake. This hormone is capable of reducing proliferation of MDA-ERα7 cell line. Studies show that ratio of gAcrp30 to leptin and the ERα (estrogen receptor) status of cells are key in determining BRCA (breast cancer) cell growth. |
| General description: | Acrp30 (adipocyte complement-related protein), or cytokine adiponectin, is a hormone produced and secreted by differentiated adipocytes. This protein is composed of an N-terminal collagenous domain and a C-terminal globular trimerization domain. It exists in plasma predominantly as trimeric, hexameric or other high-molecular weight forms. The globular subunit gAcrp30 is however absent in plasma and is biologically active. gAcrp30 is the truncated form of full length of Acrp30, and functions as a ligand of AdipoR1. Recombinant mouse gAcrp30 is a 16.6 kDa protein consisting of 145 amino acid residues. |
| Physical form: | Lyophilized from 5 mM Tris, pH 7.6. |
| Reconstitution: | Centrifuge the vial prior to opening. Avoid freeze-thaw cycles. |
| Reconstitution: | Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/mL. This stock solution can then be diluted into other aqueous buffers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |