IFN-alpha 2a human
SIGMA/SRP4594 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Synonym: B cell interferon; IFN-α 2A; IFNA2; Interferon α-2a; Leukocyte interferon; Type I interferon
MDL Number: MFCD01635115
Product Type: Chemical
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | human |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | ~19.4 kDa |
| NCBI accession no. | P01563   |
| packaging | pkg of 100 μg |
| potency | 1x10^8 units/mg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | P01563 |
| Application: | IFN-alpha 2a human has been used: • as standard protein in reverse-phase high-performance liquid chromatography (RP-HPLC) and reducing and non-reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for characterizing recombinant consensus interferon (rh cIFN) • as a supplement in Roswell Park Memorial Institute (RPMI) 1640 medium for studying interferon resistance in peripheral blood mononuclear cells (PBMC) • to treat hepatocytes to test its effect on signal transducer and activator of transcription 1 (STAT1) mRNA levels and cytochrome P450 family 1 subfamily A member 2 (CYP1A2) expression • to stimulate human monocyte derived macrophages (MDMs) |
| Application: | IFN-α2a (interferon α 2a)-human has been used to study heterosexual transmission of subtype C HIV (human immunodeficiency virus)-1. |
| Biochem/physiol Actions: | IFN-α2a (interferon α 2a) is a lymphoid factor that possesses potent anti-viral activity. It mainly interacts with cell surface-specific receptors. This interaction is responsible for anti-pathogenic response in cells. IFN-α2a also exhibits anti-tumor activities. Humans with brain tumor were shown to have mutations in this gene. Interferon-α-2a (IFN-a 2A) favors 2′5′-oligoadenylate synthetase and double-stranded RNA-dependent protein kinase induction to target viral mRNA and protein synthesis. IFN-α 2A exhibits immunomodulatory effects, inducing cytokines and other interferon production. It also increases natural killer and cytotoxic T cell activity, as well as histocompatibility complex antigen expression. It may be beneficial for treating hepatic cirrhosis, chronic hepatitis C and hepatitis D. |
| General description: | The gene IFN-α2a (interferon α 2a) is mapped to human chromosome 9. The encoded protein is a type 1 interferon and a glycoprotein. It belongs to the class of cytokines. Recombinant human IFN-α 2a is a single, non-glycosylated, polypeptide chain with a molecular mass 19.4kDa containing 166 amino acid residues. Interferon-α-2a, a recombinant interferon-a, is expressed in Escherichia coli. It is eliminated majorly via renal catabolism. However, hepatic and biliary elimination comprise the minor pathways. |
| Physical form: | Lyophilized without any additives. |
| Reconstitution: | Centrifuge the vial prior to opening. Avoid freeze-thaw cycles. |
| Reconstitution: | Reconstitute in water to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |