IFN-alpha 2b human
SIGMA/SRP4595 - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym: IFNA; INFA2; Interferon α 2b
Product Type: Chemical
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | human |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | ~19.3 kDa |
| NCBI accession no. | Q86UP4   |
| packaging | pkg of 100 μg |
| potency | >2.0x10^7 units/mg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | Q86UP4 |
| Application: | IFN-α2b human has been used: • as an immunotherapeutic agent to test its antiproliferative and proapoptotic effects in RCC cell lines • to stimulate peripheral blood mononuclear cell (PBMC) prior to RNA isolation • to stimulate H4 cells before immunoprecipitation • to study role of USP18 (ubiquitin specific peptidase 18)-mediation deISGylation in controlling cellular autophagy upon treatment with type I interferons |
| Biochem/physiol Actions: | IFN-α2b (interferon α 2b) is a lymphoid factor that possesses potent anti-viral activity. It mainly interacts with cell surface-specific receptors. This interaction is responsible for anti-pathogenic response in cells. IFN-α2b also exhibits anti-tumor activities. Humans with brain tumor were shown to have mutations in this gene. IFN-α2b induces the secretion of cytokine IFN-γ in T cells. The hydrogels embedded with IFN-α2b exploits this functionality in gastric cancer cells. Recombinant IFN-α types are effective against Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in in vitro studies. |
| General description: | The gene IFN-α2b (interferon α 2b) is mapped to human chromosome 9. The encoded protein is a type 1 interferon and a glycoprotein. It belongs to the class of cytokines. Human IFN-α2b is a 19.3kDa protein containing 165 amino acid residues. Structurally, IFN-α2b is O-glycosylated at the threonine residue at position 106. |
| Physical form: | Lyophilized from 5 mM sodium phosphate, pH 7.4. |
| Reconstitution: | Reconstitute in water to a concentration of 1 mg/mL. This solution can then be diluted into other aqueous buffers. Centrifuge the vial prior to opening. Avoid freeze-thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |