IFN-alpha 1 human
SIGMA/SRP4596 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Synonym: IFN-α 1; IFNA1; Interferon-α
MDL Number: MFCD01773209
Product Type: Chemical
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | human |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | ~19.3 kDa |
| NCBI accession no. | NM_024013   |
| packaging | pkg of 100 μg |
| potency | 1x10^8 units/mg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | P01562 |
| Application: | IFN-alpha 1 human has been used: • to treat Vero and breast cancer (MDA-MB-231) cells in interferon assay before viral infection • as a ligand to vaccinia virus B18R protein in analytical size exclusion chromatography • to treat chikungunya infected human embryonic kidney cell line (HEK293T) to test its effect on Signal transducer and activator of transcription 1 (STAT1) phosphorylation • as recombinant interferon to treat lung fibroblast cell line (IMR90) to monitor histone chaperone (HIRA) response |
| Biochem/physiol Actions: | IFN-α1 (interferon α 1) is a lymphoid factor that possesses potent anti-viral activity. IFN-α1 interacts with IFNAR1 (interferon α receptor 1) and IFNAR2. The interaction activates JAK1 (janus-activated kinase 1) and tyrosine kinase 2. These events phosphorylate and activate STAT1 and STAT2 (signal transducer and activator of transcription proteins), leading to the upregulation of interferon-stimulated genes some of which encode antiviral proteins. IFN-alpha 1 (IFN-α1) mediates apoptosis in specific tissues. It may be useful in treating laryngeal carcinoma. The interaction of microRNA, miR-181b with IFN-α1, is regarded as a risk factor in systemic lupus erythematosus. |
| General description: | IFN-α1 (interferon α 1) is a type 1 interferon. Human IFN-a1 is a 19.3kDa protein containing 166 amino acid residues. IFN-α1 belongs to the type I interferon (IFN) family and is present in leukocytes. The IFN-α1 gene is mapped to human chromosome 9p21.3. |
| Physical form: | Lyophilized without any additives. |
| Reconstitution: | Centrifuge the vial prior to opening. Avoid freeze-thaw cycles. |
| Reconstitution: | Reconstitute in water to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |