Myostatin Proform human
SIGMA/SRP4623P - recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym: GDF-8; Growth Differentiation Factor 8; MSTN; MSTN Muscle Hypertrophy
MDL Number: MFCD03457656
Product Type: Chemical
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| biological source | human |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | ~27.8 kDa |
| NCBI accession no. | O14793   |
| packaging | pkg of 10 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| Biochem/physiol Actions: | MSTN (myostatin) functions as a negative modulator of muscle cell development. It functions through forming heteromeric receptor complexes such as with activin type II receptor B (ActRIIB), and with lesser affinity with ActIIA. This interaction results in the recruitment of type I receptors like activin receptor-like kinase 4 (ALK4) or ALK5, leading to the phosphorylation of Smad2 (small mothers against decapentaplegic) and Smad3, which move to the nucleus and suppress myogenesis. In MSTN-null mice, there is delayed skin wound healing due to the increased expression of decorin, an endogenous transforming growth factor-β (TGF β) suppressor. Topical treatment with TGF-β1 restored skin wound healing, suggesting that MSTN agonists might have therapeutic beneficial in skin wound repair. |
| General description: | MSTN (myostatin) belongs to the transforming growth factor-β (TGF β) superfamily containing numerable homologous cytokine proteins. This gene is expressed in skeletal muscle, and codes for a precursor protein composed of an N-terminal leader peptide, a pro-peptide domain, and a C-terminal domain. This precursor protein is proteolytically cleaved by bone morphogenetic protein 1 (BMP-1) at a conserved four amino acid RSRR site to produce the 13kDa mature protein. |
| Physical form: | Lyophilized without any additives. |
| Reconstitution: | Centrifuge the vial prior to opening. Avoid freeze-thaw cycles. |
| Reconstitution: | Reconstitute in water to a concentration of 0.1 to 1.0 mg/mL. This solution can then be diluted into other aqueous buffers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC); ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |