Prolactin from mouse
SIGMA/SRP4688 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Synonym: Luterotropic hormone; Lutetropin; Mammotropin; PRL
Product Type: Chemical
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | mouse |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | ~22.5 kDa |
| NCBI accession no. | P06879   |
| packaging | pkg of 50 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| Application: | PRL (Prolactin) from mouse has been used to study the effects of prolactin on the length, motility and viability of larvae of Toxocara canis. |
| Application: | Prolactin from mouse has been used as a supplement in mammary epithelial basal medium (MEBM) for mammary epithelial cell culture. |
| Biochem/physiol Actions: | PRL (Prolactin) primary function is to promote and maintain lactation during pregnancy and suckling. In addition, prolactin plays an immune-regulatory role by stimulating the activities of ornithine decarboxylase and protein kinase C, which are important for the proliferation, differentiation, and function of lymphocytes. In mice, prolactin also prevents hepatocellular carcinoma by suppressing MAP3K (mitogen-activated protein kinase kinase kinase)-dependent activation of c-myc. |
| Biochem/physiol Actions: | Prolactin synthesis is regulated in the hypothalamus. Dopamine and prolactin secretion from anterior pituitary lactotrophs are controlled by a negative feedback loop. Prolactin modulates xenobiotic metabolism by controlling the mRNA expression cytochrome p450 enzymes. |
| General description: | Prolactin (PRL, also known as mammotrophin, luterotropic hormone, and lutetropin) is a neuroendocrine hormone secreted by the pituitary gland. It belongs to the cytokine receptor superfamily. Recombinant mouse prolactin is a 22.5kDa globular protein containing 198 amino acid residues. |
| Physical form: | Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 8.0 plus 50 mM NaCl. |
| Reconstitution: | Centrifuge the vial prior to opening. Avoid freeze-thaw cycles. |
| Reconstitution: | Reconstitute in water to a concentration of 1.0 mg/mL. This solution can then be diluted into other aqueous buffers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |