Prolactin from rat
SIGMA/SRP4689 - recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Synonym: Luterotropic hormone; Lutetropin; Mammotropin; PRL
Product Type: Chemical
| assay | ≥95% (HPLC) |
| ≥95% (SDS-PAGE) | |
| biological source | rat |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | ~22.5 kDa |
| NCBI accession no. | P01237   |
| packaging | pkg of 50 μg |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| Biochem/physiol Actions: | Prl (prolactin) is processed to lower molecular weight N-terminal fragments, 14-18kDa, called vasoinhibins which have antiangiogenic effects. In endothelial cells, the 16kDa prolactin form exerts antiproliferative and proapoptotic effects which result in cell cycle arrest, caspase and NFκB (nuclear factor) activation, and regulation of Bcl-2 (B-cell lymphoma 2) protein family. In sexually mature female mice, the plasma levels of prolactin remain constant throughout estrous cycle, but show a sudden increase during proestrous cycle due to increase in circulating levels of estrogens. Studies show that Prl activates T cells, B cells, natural killer (NK) cells, macrophages, neutrophils, and dendritic cells, and there is a significant level of hyperprolactinemia incidence in patients with autoimmune diseases, such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Studies in rats show that Prl is involved in T cell homing to mammary glands during early lactation, and that hypoprolactemia during lactation results in modified T cell distribution with increased T cell in circulation and reduced in mammary glands. |
| General description: | Prl (prolactin) is an anterior pituitary hormone, with a molecular weight of 23kDa. It is processed into several molecular forms such as phosphorylated, glycosylated, and lower molecular weight forms. Cleavage of the native protein results in the production of multiple N-terminal fragments of 14 to 18kDa molecular weights, known as vasoinhibins. Recombinant mouse Prolactin is a 22.5 kDa globular protein containing 198 amino acid residues. |
| Physical form: | Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 8.0 plus 50 mM NaCl. |
| Reconstitution: | Centrifuge the vial prior to opening. Avoid freeze-thaw cycles. |
| Reconstitution: | Reconstitute in water to a concentration of 1.0 mg/mL. This solution can then be diluted into other aqueous buffers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC); ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |