TSLP human
SIGMA/SRP4896 - recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC)
Synonym: Thymic Stromal Lymphopoietin
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| assay | ≥97% (HPLC) |
| ≥97% (SDS-PAGE) | |
| biological source | human |
| form | lyophilized |
| impurities | endotoxin, tested |
| mol wt | ~15.0 kDa |
| NCBI accession no. | Q969D9   |
| packaging | pkg of 20 μg |
| potency | <0.3 ng/mL ED50 |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| Application: | TSLP human has been used in immunohistochemistry. |
| Biochem/physiol Actions: | Thymic stromal lymphopoietin (TSLP) signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the interleukin-7 (IL-7) receptor a chain. TSLP impacts myeloid cells and thus induces the discharge of T cell-attracting chemokines from monocytes and increases the growth of CD11c+ dendritic cells. Similar to IL-7, TSLP enhances phosphorylation of signal transducer and activator of transcription 3 and 5 (STAT3 and STAT5), though uses kinases excluding Janus kinases (JAKs) for its activation. TSLP induces the release of T cell-attracting chemokines such as thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) from monocytes and triggers CD11c+ dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as interleukin-4, interleukin-5, interleukin-13 and tumor necrosis factor-α (TNF-α), whereas down-regulating interleukin-10 and interferon-γ (IFN-γ) play a role in the initiation of allergic inflammation. |
| General description: | Thymic stromal lymphopoietin (TSLP) is a hemopoietic cytokine. It is part of the interleukin-2 (IL-2) cytokine family. It is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities to interleukin-7 (IL-7) and binds with the heterodimeric receptor complex consisting of interleukin-7 receptor α chain & the TSLP receptor. Recombinant human TSLP produced in Escherichia coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15kDa. |
| Physical form: | Lyophilized from a concentrated solution (1 mg/ml) containing 130 mM NaCl, and 20 mM sodium phosphate, pH 7.4. |
| Preparation Note: | Centrifuge the vial prior to opening. Avoid freeze-thaw cycles. |
| Preparation Note: | Reconstitute in sterile dH2O to a concentration of 0.1 -1 mg/mL and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (HPLC); ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |