AKT1, active, GST tagged human
SIGMA/SRP5001 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MGC99656; PKB; PRKBA; RAC; RAC-α
Product Type: Chemical
SDS-PAGE AKT1, active, GST tagged, human: Cat. No. SRP5001: Purity was determined by densitometry. Molecular weight ~85 kDa. See CofA for lot-specific data.
Specific Activity AKT1, active, GST tagged, human: Cat. No. SRP5001: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~85 kDa |
| NCBI accession no. | NM_005163   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 103-139 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | v-akt murine thymoma viral oncogene homolog 1 (AKT1) is responsible for the phosphorylation and cytoplasmic localization of CKDN1A (cyclin-dependent kinase (CDK) inhibitor 1, also referred to as p21). However, p21 is one of the CDKNs which normally resides in the nucleus and its cytoplasmic localization, mediated by AKT1, is common to various cancers where it suppresses proteins essential for apoptosis. Studies show that up-regulation of AKT1 serves as a poor prognostic marker in breast cancer, which however, depends upon factors including population, hormone receptor status, and hormonal or trastuzumab therapy status. |
| General description: | v-akt murine thymoma viral oncogene homolog 1 (AKT1) is a Ser/Thr protein kinase, and is also called protein kinase B (PKB). This protein is activated when it is phosphorylated at serine 473 and threonine 308. This gene is localized on human chromosome 14q32.32. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 103-139 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |