AMPK (α2/β2/γ1), active, His tagged human
SIGMA/SRP5003 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: AMPK; AMPK2; AMPKG; MGC61468; MGC8666; PRKAA; PRKAA2; PRKAB2; PRKAG1
Product Type: Chemical
SDS-PAGE AMPK (A2/B2/G1), active, His tagged, human: Cat. No. SRP5003: Purity was determined by densitometry. Molecular weight ~69 kDa (A2), ~36 kDa (B2), ~41 kDa (G1). See CofA for lot-specific data.
Specific Activity AMPK (A2/B2/G1), active, His tagged, human: Cat. No. SRP5003: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~36 kDa (B2) |
| ~40 kDa (G1) | |
| ~69 kDa (A2) | |
| NCBI accession no. | NM_002733   |
| NM_005399 |
|
| NM_006252 |
|
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 238-322 nmol/min·mg |
| storage temp. | −70°C |
| General description: | AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKβ. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 238-322 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |