CDC25A, active, GST tagged human
SIGMA/SRP5005 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CDC25A2
Product Type: Chemical
SDS-PAGE CDC25A, active, GST tagged, human: Cat. No. SRP5005: Purity was determined by densitometry. Molecular weight ~94 kDa. See CofA for lot-specific data.
Specific Activity CDC25A, active, GST tagged, human: Cat. No. SRP5005: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~94 kDa |
| NCBI accession no. | NM_001789   |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 26-36 nmol/min·mg |
| storage temp. | −70°C |
| General description: | CDC25A (also known as cell division cycle 25 homolog A) is a member of the CDC25 family of phosphatases that are required for progression from G1 to the S phase of the cell cycle. CDC25A can activate the cyclin-dependent kinase CDC2 (also known as CDK1) by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A overexpression is detected in human cancers and this may contribute to the tumorigenesis process. CDC25A is degraded by moderate heat shock and this degradation is protected by HSP90. |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 26-36 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |