CDK1/CyclinB1, active, GST tagged human
SIGMA/SRP5009 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CCNB; CCNB1; CDC2
Product Type: Chemical
SDS-PAGE CDK1/CyclinB1, active, GST tagged, human: Cat. No. SRP5009: Purity was determined by densitometry. Molecular weight CDK1 ~58 kDa and CyclinA2 ~75 kDa. See CofA for lot-specific data.
Specific Activity CDK1/CyclinB1, active, GST tagged, human: Cat. No. SRP5009: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~58 kDa (CDK1) |
| ~75 kDa (CyclinB1) | |
| NCBI accession no. | NM_001786   |
| NM_031966 |
|
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 12-16 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | The cyclin B1 (CCNB1) and cyclin-dependent kinase 1 (CDK1) complex plays a crucial role in the regulation of mitosis. Elevated expression of CCNB1 has been observed in various types of human cancers including breast, lung, colon, prostate and head and neck cancers. Thus, this protein can be considered as a potent target for cancer vaccines.In addition, CCNB1 also serves as a prognostic biomarker for estrogen-receptor-positiv |
| General description: | The cyclin B1 (CCNB1) gene is mapped to human chromosome 5q13.2. The gene codes for a 62kDa protein containing a classical cyclin box domain. CCNB1 shuttles between the nucleus and cytoplasm until prophase begins. It is a member of the cyclin family and is expressed at low levels in normal cells. CDK1 (cyclin-dependent kinase 1) belongs to the Ser/Thr protein kinase family. The gene encoding it is localized on human chromosome 10q21. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 12-16 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |