CDK2/CyclinA1, active, GST tagged human
SIGMA/SRP5010 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: p33(CDK2)
Product Type: Chemical
SDS-PAGE CDK2/CyclinA1, active, GST tagged, human: Cat. No. SRP5010: Purity was determined by densitometry. Molecular weight CDK1 ~58 kDa and CyclinA1 ~81 kDa. See CofA for lot-specific data.
Specific Activity CDK2/CyclinA1, active, GST tagged, human: Cat. No. SRP5010: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~58 kDa (CDK1) |
| ~81 kDa (CyclinA1) | |
| NCBI accession no. | BC036346   |
| NM_001798 |
|
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 70-95 nmol/min·mg |
| storage temp. | −70°C |
| General description: | CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 70-95 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |