CDK5/p35., active, GST tagged human
SIGMA/SRP5011 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CDKN5; PSSALRE
Product Type: Chemical
SDS-PAGE CDK5/p35., active, GST tagged, human: Cat. No. SRP5011: Purity was determined by densitometry. Molecular weight CDK5 ~59 kDa and p35 ~60 kDa. See CofA for lot-specific data.
Specific Activity CDK5/p35., active, GST tagged, human: Cat. No. SRP5011: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~59 kDa (CDK5) |
| ~60 kDa (p35) | |
| NCBI accession no. | NM_003885   |
| NM_004935 |
|
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| solubility | soluble |
| water: soluble | |
| specific activity | 157-213 nmol/min·mg |
| storage temp. | −70°C |
| technique(s) | activity assay: suitable |
| Application: | CDK5 has been used as a component of a buffer during the measurement of the in vitro phosphorylation of peroxisome proliferator-activated receptor gamma (PPARγ). |
| Biochem/physiol Actions: | CDK5 is a neuron-specific kinase that plays a vital role in the development, functioning, and diseases of the central nervous system. Its optimum functioning is highly essential for proper neuronal migration, differentiation, axonal elongation, and synaptic function. CDK5 modulates neurite outgrowth, and axonal growth by interacting with several substrates like N- cadherin, Rap Guanine Nucleotide Exchange Factor 2(RapGEF2), focal adhesion kinase (FAK), p21 activating kinase 1, serotonin 6 receptor and Ras-related protein 8. CDK5 also plays a key role in the formation of the synapse, its maintenance, and communication. It is involved in the formation and retraction of the dendritic spines. It also regulates neuron secretion at the synapse by phosphorylating key mediators such as Synaspsin1. In addition, CDK5, present in the synaptic membrane phosphorylates the erythroblastic leukemia viral oncogene homolog B receptor (ErbB), resulting in increased acetylcholine receptor transcription. Lastly, CDK5 is also involved in the regulation of various other cellular processes like cell cycle, cell survival, apoptosis, gene regulation, etc. |
| General description: | Research area: Apoptosis Cdk5 is a proline-directed serine/threonine protein kinase that belongs to the family of cyclin-dependent kinases (CDKs). Active CDK5, also known as neuronal cdc2-like kinase is a heterodimer of CDK5 and is essential for its kinase activity. They are found abundantly in the mammalian brain. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 157-213 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |