CDK9/CyclinK, active, GST tagged human
SIGMA/SRP5012 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: C-2k; CCNK; CDC2L4; CPR4; MGC9113; PITALRE; TAK
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
mol wt | ~67 kDa (CyclinK) |
~68 kDa (CDK9) | |
NCBI accession no. | BC015935 ![]() ![]() |
NM_001261 ![]() ![]() |
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product line | PRECISIO® Kinase |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 15-21 nmol/min·mg |
storage temp. | −70°C |
Biochem/physiol Actions: | CDKs are closely related to yeast CDk1, cdc28 and cdc2 and arean important regulators of the cell cycle. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the human immune deficiency virus 1 (HIV-1) transactivating regulatory protein (Tat) protein, which suggests a possible involvement of this protein in AIDS. |
General description: | CDK9/CyclinK, active, GST tagged human comprises recombinant full-length human CDK9 and cyclinK proteins co-expressedin Sf9 insect cells with an N-terminal GST tag. Cyclin-dependent protein kinase 9 (CDK9)/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is a component of the multiprotein complex mitogen-activated protein kinase 7 and transcription elongation factor β (TAK/P-TEFβ). |
Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 15-21 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |