CDK9/CyclinK, active, GST tagged human
SIGMA/SRP5012 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: C-2k; CCNK; CDC2L4; CPR4; MGC9113; PITALRE; TAK
Product Type: Chemical
SDS-PAGE CDK9/CyclinK, active, GST tagged, human: Cat. No. SRP5012: Purity was determined by densitometry. Molecular weight CDK9 ~68 kDa and CyclinK ~67 kDa. See CofA for lot-specific data.
Specific Activity CDK9/CyclinK, active, GST tagged, human: Cat. No. SRP5012: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~67 kDa (CyclinK) |
| ~68 kDa (CDK9) | |
| NCBI accession no. | BC015935   |
| NM_001261 |
|
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 15-21 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | CDKs are closely related to yeast CDk1, cdc28 and cdc2 and arean important regulators of the cell cycle. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the human immune deficiency virus 1 (HIV-1) transactivating regulatory protein (Tat) protein, which suggests a possible involvement of this protein in AIDS. |
| General description: | CDK9/CyclinK, active, GST tagged human comprises recombinant full-length human CDK9 and cyclinK proteins co-expressedin Sf9 insect cells with an N-terminal GST tag. Cyclin-dependent protein kinase 9 (CDK9)/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is a component of the multiprotein complex mitogen-activated protein kinase 7 and transcription elongation factor β (TAK/P-TEFβ). |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 15-21 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |