CK1 α 1, active, GST tagged human
SIGMA/SRP5013 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CK1; CK1A1; CSNK1A1; HLCDGP1; PRO2975
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
mol wt | ~62 kDa |
NCBI accession no. | NM_001892 ![]() ![]() |
product line | PRECISIO® Kinase |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 102-138 nmol/min·mg |
storage temp. | −70°C |
Application: | GST tagged active CK1α1 has been used in downstream coprecipitation and mass spectrometry to demonstrate the phosphorylation of the Wnt regulator, Jade-1, by CK1α1. |
General description: | Casein kinase 1 α1 (CK1α1) is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes. CK1α1 can regulate Smo cell surface accumulation and activity in response to hedgehog. CK1α1 phosphorylates Smo at several sites and phosphorylation-deficient forms of Smo fail to accumulate on the cell surface and are unable to transduce the hedgehog signal. CK1α1 dynamically associates with the CARMA1-BCL10-MALT1 (CBM) complex on T cell receptor engagement to participate in cytokine production and lymphocyte proliferation. CK1α1 can form complex with the E3 ubiquitin-protein ligase MDM2, which then regulates the stability of p53 and E2F-1 transcription factors. |
Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 102-138 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |