CK1 δ, active, GST tagged human
SIGMA/SRP5014 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CK1D; CSNK1D; HCKID
Product Type: Chemical
SDS-PAGE CK1 delta, active, GST tagged, human: Cat. No. SRP5014: Purity was determined by densitometry. Molecular weight ~72 kDa. See CofA for lot-specific data.
Specific Activity CK1 delta, active, GST tagged, human: Cat. No. SRP5014: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~72 kDa |
| NCBI accession no. | NM_001893   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 70-94 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | CK1δ is a regulator of Yes-associated protein (YAP) transcription coactivator, which is a key regulator of organ size and a candidate human oncogene. CK1d is activated by CCK2R and it then phosphorylates polycystin 2 (PKD2) at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells. CKId can phosphorylate in vitro deoxycytidine kinase (dCK), which is a key enzyme in the salvage of deoxynucleosides. Phosphorylation of dCK by CKId in vitro correlates with increased activity of this enzyme. |
| General description: | Casein kinase 1 δ (CK1δ) is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 70-94 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |