CK1 γ 2, active, GST tagged human
SIGMA/SRP5016 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CK1G2; CSNK1G2
Product Type: Chemical
SDS-PAGE CK1 gamma 2, active, GST tagged, human: Cat. No. SRP5016: Purity was determined by densitometry. Molecular weight ~73 kDa. See CofA for lot-specific data.
Specific Activity CK1 gamma 2, active, GST tagged, human: Cat. No. SRP5016: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~73 kDa |
| NCBI accession no. | NM_001319   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 25-35 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | Casein kinase 1 γ 2 (CK1γ2) can interact and phosphorylate the metastatic tumor antigen 1 short form (MTA1s) co-localizing it in the cytoplasm. Phosphorylated MTA1s then sequesters the estrogen receptor-α in the cytoplasm of breast cancer cells. CK1γ2 hyperphosphorylates the ceramide transfer protein CERT leading to a decrease in de novo sphingomyelin synthesis. The reduction in synthesis of sphingomyelin is reversed by the expression of CERT mutants that are not hyperphosphorylated. |
| General description: | Casein kinase 1 γ 2 (CK1γ2) is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. It is a ubiquitously expressed cytoplasmic kinase. The gene encoding this protein is localized on human chromosome 19p13.3. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 25-35 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |