CK1 γ 2, active, GST tagged human
SIGMA/SRP5016 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CK1G2; CSNK1G2
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
mol wt | ~73 kDa |
NCBI accession no. | NM_001319 ![]() ![]() |
product line | PRECISIO® Kinase |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 25-35 nmol/min·mg |
storage temp. | −70°C |
Biochem/physiol Actions: | Casein kinase 1 γ 2 (CK1γ2) can interact and phosphorylate the metastatic tumor antigen 1 short form (MTA1s) co-localizing it in the cytoplasm. Phosphorylated MTA1s then sequesters the estrogen receptor-α in the cytoplasm of breast cancer cells. CK1γ2 hyperphosphorylates the ceramide transfer protein CERT leading to a decrease in de novo sphingomyelin synthesis. The reduction in synthesis of sphingomyelin is reversed by the expression of CERT mutants that are not hyperphosphorylated. |
General description: | Casein kinase 1 γ 2 (CK1γ2) is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. It is a ubiquitously expressed cytoplasmic kinase. The gene encoding this protein is localized on human chromosome 19p13.3. |
Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 25-35 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |