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DAPK2, active, GST tagged human

SIGMA/SRP5018 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: DRP-1; MGC119312

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SRP5018-10UG 10 µg
$518.00
1/EA
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SDS-PAGE DAPK2, active, GST tagged, human: Cat. No. SRP5018: Purity was determined by densitometry. Molecular weight ~67 kDa. See CofA for lot-specific data.
Specific Activity DAPK2, active, GST tagged, human: Cat. No. SRP5018: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.

 

assay ≥70% (SDS-PAGE)
form buffered aqueous glycerol solution
mol wt ~67 kDa
NCBI accession no. NM_014326 
product line PRECISIO® Kinase
recombinant expressed in baculovirus infected Sf9 cells
shipped in dry ice
specific activity 115-156 nmol/min·mg
storage temp. −70°C
General description: DAPK2 or death-associated protein kinase 2 belongs to a family of proapoptotic Ca(2+)/calmodulin-regulated serine/threonine kinases. Overexpression of DAPK2 induces cell apoptosis. DAPK2 has been shown to be a novel Sp1-dependent target gene for E2F1 and Krüppel-like factor 6 (KLF6) in cell death response. Both E2F1 and KLF6 strongly activate the DAPK2 promoter. DAPK2 plays a role in granulopoiesis where it is highly expressed. β-catenin can block anoikis of malignant kidney and intestinal epithelial cells and promote their anchorage-independent growth by down-regulating DAPK2. β-catenin-induced down-regulation of DAPK2 requires the presence of the transcription factor TCF-4.
Legal Information: PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
Physical form: Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note: after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥70% (SDS-PAGE)
activity specific activity: 115-156 nmol/min·mg
Storage Temp. −70°C
UNSPSC 12352200

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