GRK7, active, GST tagged human
SIGMA/SRP5036 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: GPRK7
Product Type: Chemical
SDS-PAGE GRK7, active, GST tagged, human: Cat. No. SRP5036: Purity was determined by densitometry. Molecular weight ~89 kDa. See CofA for lot-specific data.
Specific Activity GRK7, active, GST tagged, human: Cat. No. SRP5036: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~89 kDa |
| NCBI accession no. | NM_139209   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 2.1-2.9 nmol/min·mg |
| storage temp. | −70°C |
| General description: | GRK7 is a member of the G protein-coupled receptor kinase subfamily and is a Ser/Thr protein kinase. GRK7 is specifically expressed in the retina and has been shown to phosphorylate cone opsins and initiate their deactivation. GRK7 catalyzes rhodopsin phosphorylation in a light-dependent manner. GRK7 co-localizes with GRK1 in human cone outer segments. PKA phosphorylates GRK7 at Ser(23) and Ser(36) in vitro and phosphorylation of GRK7 by PKA reduces the ability of GRK7 to phosphorylate rhodopsin in vitro. Since exposure to light causes a decrease in cAMP levels in rod cells, it is proposed that phosphorylation of GRK7 by PKA occurs in the dark. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 2.1-2.9 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |