IKK α , active, GST tagged human
SIGMA/SRP5040 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CHUK; IKBKA; IKK-α; IKK1; NFKBIKA; TCF16
Product Type: Chemical
SDS-PAGE IKK alpha , active, GST tagged, human: Cat. No. SRP5040: Purity was determined by densitometry. Molecular weight ~114 kDa. See CofA for lot-specific data.
Specific Activity IKK alpha , active, GST tagged, human: Cat. No. SRP5040: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~114 kDa |
| NCBI accession no. | BC092514   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 2.1-2.9 nmol/min·mg |
| storage temp. | −70°C |
| General description: | IKKα is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. IKKα is an essential regulator of NF-kappa-B-dependent gene expression through control of promoter-associated histone phosphorylation after cytokine exposure. IKKα is a critical component of the cytoplasmic transductional-transcript |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 2.1-2.9 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |